Telomere-to-telomere sequencing (T2T) on PromethION (SQK-APK114, SQK-LSK114, and SQK-ULK114)
| Date | Version number | Language | Device | Changes | |
|---|---|---|---|---|---|
| Nov 26 2024 | T2T_9211_v114_revF_27Nov2024 | English | PromethION | 27th November 2024 - SQK-ULK114 kit content updated with new kit layout and Precipitation Star (PS) information. - In the Ultra-long sequencing experiment: added optional step for use of Precipitation Star (PS) in clean-up following DNA tagmentation. | |
| Nov 1 2024 | T2T_9211_v114_revE_04Sep2024 | English | PromethION | 1st November 2024 - Updated "Overview of the protocol": Removal of reference to Herro within the "Sequencing and analysis" section in reference to "Assembly Polishing Kit experiment". Now references use of Dorado for read correction. - Included instructions for obtaining and installing the SQK-APK114 sequencing script on your device in the "Data acquisition and basecalling: Assembly Polishing Kit" section of the protocol. - Updated formatting for the "Data acquisition and basecalling" pages of the protocol to more easily separate out the parameters of each section. | |
| Nov 1 2024 | T2T_9211_v114_revD_04Sep2024 | English | PromethION | 1st November 2024 - Updated "Overview of the protocol": Removal of reference to Herro within the "Sequencing and analysis" section in reference to "Assembly Polishing Kit experiment". Now references use of Dorado for read correction. - Included instructions for obtaining and installing the SQK-APK114 sequencing script on your device in the "Data acquisition and basecalling: Assembly Polishing Kit" section of the protocol. - Updated formatting for the "Data acquisition and basecalling" pages of the protocol to more easily separate out the parameters of each section. | |
| Sep 24 2024 | T2T_9211_v114_revC_04Sep2024 | English | PromethION | 24th September 2024 - Medaka model pinned to version 1.12.1 to avoid discrepancy issues in downstream analysis. | |
| Sep 10 2024 | T2T_9211_v114_revB_04Sep2024 | English | PromethION | 10th September 2024 - Updated setting recommendations in downstream analysis. | |
| Sep 9 2024 | T2T_9211_v114_revA_04Sep2024 | English | PromethION | 09th September 2024 Protocol release | |
| Sep 6 2024 | Version2a_Rev14 | English | PromethION | 04th September 2024 Protocol release | |
| Aug 30 2024 | Version2a_Rev13 | English | PromethION |