Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.
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The “Channels Panel” reflects the real-time channel states.
Different states are represented by different colours.
Below is an example of the Channels Panel of a MinION sequencing run.
• Sequencing (light green): a DNA strand translocating through a single pore.
• Pore (dark green): a single open pore that is not currently capturing DNA.
• Recovering (dark blue): a single pore with a stalled DNA strand that is no longer translocating, or a current range that is too high to be meaningful.
• Inactive (light blue): a channel that cannot be rescued and will no longer be able to sequence. Reasons can include the current being outside the detector limit or the presence of multiple pores.
• Unclassified (white): a channel that MinKNOW has not yet classified into one of the states described above.
You can click the “More” button to reveal a more detailed array of channel states:
• Strand (light green): DNA is currently passing through a single pore.
• Adapter (straw yellow): The pore is sequencing the unligated sequencing adapter only. Reads will initially be classified as adapter until the DNA/RNA strand starts translocating through the pore and MinKNOW™ is able to reclassify the read. Please note that when sequencing shorter fragments, MinKNOW™ might only label it as adapter despite there being read data in the raw signal and that this will still be basecalled.
• Single pore (dark green): Single pore is present, however, no DNA or other sample is currently passing through the pore.
• Unavailable (Turquoise): Single pore which is currently blocked. Pores can be unblocked during the course of the experiment by the software/electronics.
• Active feedback (purple): The channel is being flicked, either as part of a routine flick or to remove a stalled strand.
•Possible Multiple (orange): More than one pore is present at that sensor.
• Saturated (black): This indicates that the particular sensor was passing too much current and the channel was switched off by the software in order to not affect neighboring channels.
• Out of Range 1 (grey): Some negative current passing through that particular sensor.
• Out of Range 2 (light blue): Some current passing through that particular sensor, but the cause of the current cannot be classified (hasn't been assigned as a pore or as strand data).
• Zero (Dark blue): Very little current passing through that particular sensor (viable pore not present).
The default channel state is in olive brown, indicating a channel state hasn't been assigned for that channel yet.
For more details on the MinKNOW GUI, please see the MinKNOW technical document.