Support
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Software:
- My experiment randomly stopped acquiring data during the run
- How do I check if my pod5 file was generated using the 4 kHz or 5 kHz sampling rate?
- What version of MinKNOW was my pod5 file generated with?
- What folder are my reads in?
- What data are in the pings sent to Oxford Nanopore?
- Can I get my sequencing data in FASTQ format?
- Where can I find out more about quality scores?
- What is basecalling?
- What is a fast5 file?
- Recover raw reads for Windows
- Recover raw reads for Linux, Mac, and Mk1C
- How can I demultiplex barcoded samples?
- What is a consensus sequence?
- How can I view and interact with fast5 files?
- What should I do if I want to enrich for only a single genome by using adaptive sampling?
- What factors affect whether I will see an improvement with adaptive sampling?
- What can I try if I am not getting my entire region of interest enriched when using adaptive sampling?
- What can I do with adaptive sampling (formerly referred to as "read until")?
- If I also want to align to my genome of interest when adaptive sampling is on, do I have to specify alignment as well?
- How many more bases on target can I get when using adaptive sampling?
- Why do my files end '.fast5.tmp'?
- Do you have any recommendations for how to move data off the PromethION 24/48 in real time during a sequencing run?
- Why are so many of my reads "unclassified" by WIMP?
- What is read alignment?
- Should I QC my data and what tools to use?
- Should I filter my reads before analysis?
- Can I detect structural variation (SV)?
- Can I detect SNP/indels (insertions and deletions)?
- Can I detect modified bases?
- What tools are available for assessing the performance of the Cas9 targeted sequencing experiment?
- What is the expected coverage for the region of interest from Cas9 target sequencing and how can it be assessed?
- What is genomic sequence assembly?
- What is assembly polishing (consensus improvement)?
- How do I analyse transcriptomic data?
- Why do my reads end up in the “fast5_skip”, “queued_reads” or “tmp” folder?
- My flow cells are loaded into the PromethION, but I cannot select them in the GUI. They have stayed greyed out?
- How do I get access to the GitHub repositories?
- Can I run multiple MinIONs in parallel?
- What is the cost of the software?
- Why do I get a “CUDA_ERROR_OUT_OF_MEMORY” error when running Guppy with GPU?
- How can I obtain a Registration Code?
- Does Metrichor utilise customer uploads for its own purposes?
- What should I do if translocation speed goes up?
- Why do I get a "file operation is still pending" message asking me to restart when I try to install MinKNOW?
- Is there a way to view my Mk1C sequencing run on a bigger screen?
- How do I export logs from the Mk1C?
- How do I change the directory for the reads folder?
- Why is the Experiment and Samples upload method showing no folders in EPI2ME?
- How do I upgrade the software on GridION?
- How do I open the Agent 3.0 when using Ubuntu?
- How many flow cells can I basecall in real time on the PromethION?
- Can data from Oxford Nanopore's sequencing devices be analysed and visualized in real-time?
- At what point of the sequencing should we use the Desktop Agent for EPI2ME?
- What's the minimum fragment length that can be used with WIMP?
- How to use a sample sheet (.csv file) to fill out sample names in MinKNOW
- How do I enable the wifi hotspot on the Mk1C?
- Where is the sequencing_summary.txt file located?
- How I can determine the number of reads that are being rejected due to adaptive sampling?
- How much SSD space will my run take up?
- How can I obtain the MAC address of my Mk1C device?
- What does the “error while loading shared libraries: libcuda.so.1: cannot open shared object file” message mean when running the…
- How do I change the Q-score filter when running stand-alone Guppy?
- How do I enable live GPU basecalling on MinION Mk1B?
- How do I export a run report for a sequencing run?
- What are the network requirements to connect remotely to my sequencer via Connection Manager?
- Changing the directory for the reads folder
- Log Files
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Getting started
Your first experiment
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Find out moreManaging your devices and account
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