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16S Barcoding Kit (SQK-RAB204 and SQK-16S024)
The 3' flanking sequence of the forward primer contains a wobble base (denoted by M; in the primer the base is either an A or a C) in a variable region of the 16S gene.
Forward 16S primer:
5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'
Reverse 16S primer:
5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'