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Library prep:
- How important are the Eppendorf DNA LoBind® 1.5 ml tubes?
- What does it mean if pores are becoming saturated?
- What are the 16S sequencing primers?
- What is the concentration of Lambda?
- Is the barcoding kit compatible with cDNA libraries?
- Which protocol should I use?
- What is DNA CS (DCS)?
- Can I skip the end-prep step?
- What is the best stop point during the library preparation step?
- What are the recommended extraction methods?
- What are the adapter sequences used in the kits?
- How much adapter should I use for short amplicons?
- Can I use alternative equipment and consumables to the ones recommended in the protocol?
- Are there alternate shearing methods to the Covaris g-Tube for gDNA fragmentation?
- When working with long fragments of DNA, do I have to use wide board tips?
- Do I have to use SPRI beads for the purification step during library prep?
- Can I use small fragments of DNA with the Rapid Sequencing Kit?
- Can I use more DNA than the amount indicated in the protocol?
- What are the tailing primers sequences?
- What are the primer sequences used in the Direct RNA, PCR cDNA, and Direct cDNA Kits?
- Do freezing and thawing cycles affect DNA stability?
- Can total RNA be used as input with the RNA or cDNA kits?
- Can RNA be stored prior to sequencing?
- Where do I find protocols?
- What is the minimum gDNA input?
- How important is the quality of my DNA/RNA?
- What is the purpose of the SFB (Short Fragment Buffer) Expansion kit (EXP-SFB001)?
- What is the purpose of the Ligation Sequencing Kit XL (SQK-LSK109-XL)?
- What is the Native Barcoding Expansion kit 96 (EXP-NBD196)?
- Is the barcoding kit compatible with cDNA libraries?
- Why do we use two pools of probes instead of just one on the tiling method?
- Why are four cuts recommended for the Excision approach?
- What should I do if a white precipitate appears when adding the adapter ligation mix to the dA-tailed DNA?
- Can the ribonucleoprotein (RNP) complex be stored?
- What is the RNA integrity number (RIN)?
- What is the effect of the presence of rRNA on RNA/cDNA library preparation?
- Why do I observe a decrease in throughput when preparing long DNA library fragments?
- What’s new in SQK-LSK110 and what’s the same as in SQK-LSK109?
- What is the difference between LIB and LIS in the protocols for SQK-LSK114?
- My library preparation method requires 3rd party reagents, how many reactions worth should I order?
- What are the features of the new wash kit EXP-WSH004?
- What are the features of the Rapid Barcoding Kit 96 (SQK-RBK114.96)?
- What are the features of the Flongle Sequencing Expansion (EXP-FSE002)?
- What are the features of the Native Barcoding Auxiliary Kit V14 (EXP-NBA114)?
- What are the features and protocol for the Ultra-Long DNA Sequencing Kit (SQK-ULK114)?
- Why do I have low pore occupancy?
- What are the features of Flow Cell Wash Kit XL (EXP-WSH004-XL)?
- What is new with EXP-AUX003?
- What are the key improvements in PCR-cDNA Sequencing Kit (SQK-PCS111)?
- Can I store my DNA library?
- Do I have to run CTC and Lambda Control Experiment?
- What is pore occupancy and how to improve it?
- What is RNA CS (RCS)?
- What is the Lambda DNA control (LMD)?
- The difference between LA and NA
- Information on the SQK-LSK109 and SQK-LSK110 kits
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