Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.
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We highly recommend performing dephosphorylation of the genomic DNA step (~15 min) to remove the 5’ phosphates from the ends of the genomic DNA.
This step will help reducing the ligation of sequencing adapters to non-target DNA therefore background reads will be heavily reduced.