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Library prep: Cas9
- Which Cas9 targeting approach should I use?
- What’s the purpose of the dephosphorylation of the genomic DNA in Cas9 targeted sequencing?
- What enables CRISPR/Cas9-mediated targeting and cleavage?
- What are the storage conditions for crRNAs and tracrRNAs?
- What are the requirements of the input DNA for a Cas9-targeted sequencing experiment?
- What are the QC steps for the Cas9 targeted sequencing experiment?
- How is enrichment of the Region of Interest (ROI) achieved through the Cas9 targeted sequencing experiment?
- How does Cas9 targeted sequencing work?
- Can the region of interest (ROI) be shorter than 3kb for Cas9 sequencing?
- What is the recommended cleavage time for the Cas9 targeted sequencing?
- What are the features of the Cas9 Sequencing Kit (SQK-CS9109)?
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