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24-hour genome: end-to-end workflow from blood to analysis

This protocol aims to rapidly produce libraries with a read N50 of ~30 kb and generate ≥30x coverage of the genome, thereby providing sufficient data to robustly call small and ...

Hereditary cancer panel (HCP)

This is an end-to-end method outlining sample extraction, library preparation, sequencing, and data analysis.

Human variation sequencing from saliva samples using SQK-LSK114

This protocol describes an end-to-end process to prepare and sequence gDNA from human saliva samples, and analyse the data using the wf-human-variation workflow in EPI2ME.

Chromatin accessibility sequencing from cell samples using SQK-LSK114

This protocol describes an end-to-end process to prepare and sequence gDNA for chromatin accessibility from cell samples, and to analyse the data using the information outlined in our ...

Human cfDNA singleplex sequencing from blood using SQK-LSK114

This protocol describes how to carry out preparation and sequencing of a human cfDNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). Typically, we obtain ~50 Gb of aligned ...

Human cfDNA multiplex sequencing from blood using SQK-NBD114.24

This protocol describes how to carry out preparation and sequencing of 12 human cell-free DNA (cfDNA) samples using the Native Barcoding Kit 24 V14 (SQK-NBD114.24). Typically, we ...

Amplicon sequencing from DNA using SQK-RBK114 (.24 or .96)

This is the fastest and simplest protocol to sequence amplicon DNA. For Research Use Only Document version: RAA_9198_v114_revM_17Oct2025

Telomere multiplex sequencing (Telo-seq) from DNA using EXP-NBA114, EXP-ULA001, EXP-LFB001 and EXP-AUX003

This protocol describes how to carry out native telomere sequencing using the platform developed by Oxford Nanopore Technologies. Telo-Seq delivers single-telomere resolution at high throughput, enabling the multiplexing ...

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