Workflow: Genomic and epidemiological monitoring of yellow fever virus transmission potential
13th September 2018 -
Yellow fever (YF) is an acute viral haemorrhagic disease responsible for up to 60,000 human deaths annually. The disease is caused by a member of the Flaviviridae family with transmission primarily through mosquito species that feed on non-human primates. The MinIONTM was used in this investigation to characterise genetic diversity and monitor transmission of the virus in real-time, allowing more effective disease surveillance and outbreak control strategies to be implemented1.
This workflow presented here, as described by Faria et al1 for yellow fever virus (YFV), has been applied to other viruses including Ebola and Zika. More detailed protocol information can be found in Quick et al.2