Workflow: A complete nanopore-only assembly of an XDR Mycobacterium tuberculosis
13th September 2018 -
With over 10 million new cases reported each year and an increasing incidence of drug resistant infections, tuberculosis (TB) is one of the leading global threats to human health. To better understand the genomic changes that facilitate the emergence and spread of drug resistant M. tuberculosis strains (the predominant cause of TB), researchers at the University of Queensland, Australia, utilised nanopore sequencing to deliver a complete de novo genome assembly of a highly-transmissible XDR strain from Papua New Guinea1.
The MinION allowed the full drug-resistance profile to be determined with complete phenotypic concordance. Novel insights were also gained on GC-rich and repetitive genes that are intractable to traditional short-read sequencing technologies.