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Kim Judge

Sanger sequencing: Using GridION for large genomes in a core facility

About Kim Judge

Kim graduated with a degree in Natural Sciences from the University of Cambridge, and joined Illumina as a research associate in sequencing R&D, where she worked on the MiSeq, NextSeq500 and Nextera. She moved to the Department of Medicine at Addenbrooke’s Hospital, Cambridge in 2012. Her PhD focused on using the Oxford Nanopore MinION for microbiological applications including detecting antimicrobial resistance genes and identifying plasmids. She joined the sequencing R&D team at Sanger in 2016 where she works with the MinION, GridION and PromethION.

Abstract

Long read data has been shown to be key in producing highly contiguous assemblies for larger genomes, yet working with multiple large genomes using MinION has challenges. This presentation will discuss implementing the GridION in the Sanger Institute’s production sequencing facility, including the development of new LIMS, DNA quality assessment using FEMTO Pulse and data management and storage. I will introduce the Sanger Institute’s work with MinION and GridION sequencing for the Vertebrate Genome Project, which ultimately aims to sequence a representative of every vertebrate species. Phase one of the project aims to create reference quality haplotype-phased assemblies of selected species representing all vertebrate orders.
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