Kim Judge - Sanger sequencing: Using GridION for large genomes in a core facility
Nanopore Community Meeting 2017
Long read data has been shown to be key in producing highly contiguous assemblies for larger genomes, yet working with multiple large genomes using MinION has challenges. This presentation will discuss implementing the GridION in the Sanger Institute’s production sequencing facility, including the development of new LIMS, DNA quality assessment using FEMTO Pulse and data management and storage. I will introduce the Sanger Institute’s work with MinION and GridION sequencing for the Vertebrate Genome Project, which ultimately aims to sequence a representative of every vertebrate species. Phase one of the project aims to create reference quality haplotype-phased assemblies of selected species representing all vertebrate orders.