Aaron Pomerantz - Real-time DNA barcoding in a remote rainforest using nanopore sequencing
Nanopore Community Meeting 2017
Advancements in portable scientific instruments provide promising avenues to expedite field work in order to understand the diverse array of organisms that inhabit our planet. We tested the feasibility for in situ molecular analyses of endemic fauna using a portable laboratory fitting within a single backpack, in one of the world’s most imperiled biodiversity hotspots: the Ecuadorian Chocó rainforest. Here we demonstrate that nanopore sequencing can be implemented in a remote tropical field site to quickly and accurately identify species using DNA barcoding, as we generated consensus sequences for species resolution with an accuracy of >99% in less than 24 hours after collecting specimens. Overall, we establish how mobile laboratories and nanopore sequencing can help to accelerate species identification to aid in conservation efforts and be applied to research facilities in developing countries to promote local capacity building.