Fig. 2 Analysis report of sequence-capture data for the human exome
We have released an updated analysis workflow for sequence-capture experiments. To illustrate this, we captured the human exome using Agilent’s SureSelect Human All Exon V6 panel, and generated ~ 2.35 Gb of 1D sequence data from a MinION run, representing ~40x average coverage. We analysed the data using the resequencing analysis workflow (Fig. 2). Following basecalling, reads are mapped to the human exome reference sequence. Individual gene information is displayed, including coverage and read-accuracy distribution at that position. Future releases of this application will support the uploading of target regions, highlight known SNPs in the target regions and allow those SNPs to be displayed with a confidence value.