Workflow: Bacterial genome assembly
Assembling bacterial genomes using long nanopore sequencing reads
In order to understand the true diversity and biology of microorganisms, producing fully annotated, complete genomes is essential. However, 90% of bacterial genomes are predicted to be incomplete.
Long, PCR-free nanopore sequencing reads enable the assembly of complete, reference-quality microbial genome sequences. Nanopore sequencing shows a lack of bias in GC-rich regions, in contrast to other sequencing platforms, and can span repeat-rich sequences and structural variants that are inaccessible to traditional sequencing technologies.
Here we present a simple workflow for bacterial genome assembly from a single-organism culture, using MinION Flow Cells on MinION or GridION sequencing devices.