Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
View all Applications
Resources Investors Careers News About Store Community Contact

Workflow: Bacterial genome assembly


Date: 7th September 2022

Assembling bacterial genomes using long nanopore sequencing reads

In order to understand the true diversity and biology of microorganisms, producing fully
annotated, complete genomes is essential. However, 90% of bacterial genomes are predicted
to be incomplete.

Long, PCR-free nanopore sequencing reads enable the assembly of complete, reference-quality
microbial genome sequences. Nanopore sequencing shows a lack of bias in GC-rich
regions, in contrast to other sequencing platforms, and can span repeat-rich sequences
and structural variants that are inaccessible to traditional sequencing technologies.

Here we present a simple workflow for bacterial genome assembly from a single-organism
culture, using MinION Flow Cells on MinION or GridION sequencing devices.

Download the bacterial genome assembly workflow

Recommended for you

Open a chat to talk to our sales team