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Protocol library
Protocol library The protocol library is a comprehensive database containing over 86 Oxford Nanopore Technologies protocols for step-by-step experimental guidance. All applications are covered, from whole genome sequencing and metagenomics to cDNA and RNA sequencing. In addition, software protocols are provided for installation, configuration, and troubleshooting guidance.
Protocol builder
Protocol builder Plan your entire experiment, end-to-end, using our interactive protocol builder. Starting with your experimental aim, through to extraction protocols, library preparation, and experimental analysis, the protocol builder simplifies the experimental process, and provides a complete workflow tailored to your experimental goals and needs.
Protocol selector
Protocol selector The protocol selector provides detailed library preparation guidance. Obtain specific recommendations for library preparation based upon your sample type, input quantity, and experimental priorities.
Extraction methods
Extraction methods Extract DNA from a range of different biological samples, including plants, animal tissue, cell lines, soil, FFPE samples, and bacteria. Detailed information sheets and specific recommendations are provided for various tissue types. In addition to extraction protocols, we have prepared a collection of useful tips and tricks, including advice on RNA QC, the effects of contaminants, and cell storage, to help you get the best results from your nanopore sequencing experiment.
Sign in, or register as a guest, to access the Knowledge section within the Nanopore Community Here you will find a wide range of resources for end-to-end experimental planning, from extraction to analysis.
Bioinformatics resource Our new bioinformatics resource provides a range of in-depth guides for analysing nanopore sequencing data. Here you will find a range of in-depth analysis tutorials with step-by-step instructions and example data, including:
  • Differential transcript usage and gene expression analyses using pipeline-transcriptome-de
  • Annotation of gene transcripts and novel gene isoform discovery with pinfish
  • Differential gene expression (DGE) analysis with DESeq2
  • Review of sequencing summary statistics based on the sequencing_summary.txt file, produced by the Guppy basecalling software, with tutorial BasicQC
  • Evaluation of read-mapping characteristics from a Cas-mediated PCR-free enrichment
These tutorials require a computer workstation running a Linux operating system (Centos7, Ubuntu 18_10, Fedora 29), with the exception of the DGE analysis pipeline which is also validated on MacOS (Mojave); and they require familiarity with the command line.