Products & Services
Applications

Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
View all Applications
Resources
News Explore
Careers Contact

Eilis Hannon

Evaluation of nanopore sequencing for epigenetic epidemiology: a comparison with DNA methylation microarrays

About Eilis Hannon

Eilis Hannon is a Senior Research Fellow in the Complex Disease Epigenetics Group at the University of Exeter Medical School. She studied Mathematics as an undergraduate and holds a Ph.D. in Bioinformatics. Her research focuses on integrating multiple layers of genomic data from a range of technologies to model the dynamic nature of gene expression and epigenetic variation, and explore how this mediates genetic risk for complex diseases.

Abstract

Third-generation sequencing technologies, such as nanopore sequencing, have the potential to revolutionise the generation of epigenetic data by providing genuine genome-wide coverage. Here, we assess the viability of nanopore sequencing for epidemiology by performing a comparison with DNA methylation quantified using the most comprehensive microarray available. To attempt to rediscover genomic positions known to be differentially methylated in tobacco smokers, we implemented a CRISPR/Cas9 targeted sequencing approach, followed by nanopore sequencing. In summary, we detected smoking-associated hypomethylation at multiple sites, including within the AHRR gene, highlighting the viability of nanopore sequencing in epigenetic epidemiology.

Eilis Hannon

Eilis Hannon

Back
Open a chat to talk to our sales team