Assembly of large genomes using Oxford Nanopore and Illumina data
We have recently developed hybrid assembly methods that use a combination of short and long reads to produce remarkably high-quality assemblies from whole-genome shotgun data. The new methods are integrated in the MaSuRCA assembler system. In this talk I will describe our recent algorithm developments and their use in assembling a pathogenic fungus genome (37 Mb) and the English walnut tree genome (600 Mb), both of which produced highly contiguous genome assemblies. I will also describe our plans to to use Oxford Nanopore sequencing as part of our strategy to assemble the very challenging mega-genomes of two of the largest trees on Earth: the giant sequoia, Sequoiadendron giganteum (11 Gb) and the Coast Redwood, Sequoia sempervirens (33 Gb).