Products & Services


Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
View all Applications
News Explore
Contact Get started
Resource Centre

Ida Höijer

Amplification-free long-read nanopore sequencing for detection of Cas9 off-target activity

About Ida Höijer

Ida Höijer is a Ph.D. candidate from SciLifeLab, Uppsala University in Sweden. Prior to starting her Ph.D., Ida worked for the National Genomics Infrastructure (NGI), which is a Swedish national center for massively parallel sequencing and genotyping. At NGI she gained a lot of experience with NGS, with a focus on long-read sequencing. Ida’s Ph.D. work is centered around targeted long-read sequencing methods and their applications in medical and clinical genomics.


CRISPR-Cas9 induced off-target effects is a heavily debated concern while performing genome editing, and the prediction and detection of these are challenging. To address this issue, we developed an amplification-free long-read sequencing method for detection of Cas9 off-target activity in vitro. We call this method Nanopore off-target sequencing (Nano-OTS). Nano-OTS can detect Cas9 cleavage sites for one or several gRNAs in individual DNA samples, and our results show that a number of these sites are not reported by Cas9 off-target prediction software. Importantly, Nano-OTS can also detect Cas9 cleavage sites in “dark” genomic regions, where short-reads fail to align.

Watch here

Ida Höijer

Ida Höijer

Open a chat to talk to our sales team