Rapid sequencing with MinION
NEW! 10 minute library preparation kit now available
- Simple library prep – a two step method within 10 minutes
- Designed for rapid and efficient analysis when combined with real time data streaming
- Uses minimal additional lab equipment and consumables
- Recommended input of 200 ng high molecular weight genomic DNA (>30 kb)
- Sufficient reagents to prepare six libraries
- Designed for 1D sequencing
Simple library preparation
Two-step, 10 minute protocol producing 1D reads by sequencing one strand of the double-stranded fragment. It is a simple protocol requiring a small number of pipetting steps and a couple of incubation steps. The Rapid Sequencing Kit contains sufficient reagents to generate six libraries.
Work with less abundant samples
The exact amount of sample available for a particular experiment is variable and this library preparation has been developed to require only 200 ng of high molecular weight input DNA. Initially it is recommended that the DNA used is > 30 kb as the tagmentation process produces fragments smaller than the starting material. Varying the amount of DNA added or the ratio of DNA to transposase will alter the range of fragments produced.
The graph below shows the development of accuracy with the Rapid Sequencing Kit coupled with the R9 nanopore compared to the previous chemistries. To hear how the improvements have been made and all the other developments in this fast moving technology view the talk by Clive Brown, CTO of Oxford Nanopore.
The MinION weighs under 100g and can fit in a pocket. It is powered by the USB port on a laptop and is uniquely transportable into the field. With library preparation possible using only a minimum of equipment, field-based analysis labs have been setup in remote destinations. Combined with the portability of the MinION the workflow for this library prep method is suited to use in traditional labs and remote locations.
What is a 1D read and what is a 2D read?
1D read refers to any one strand of the DNA fragments in the library being sequenced as it translocates through the nanopore. The nanopore will sequence any strand presented to it and once the strand has passed through the pore, the pore is ready for another strand to enter.
2D read refers to both the template and complement strand being sequenced as it translocates through the nanopore; two looks at the same DNA fragment. This is facilitated by a hairpin adapter added to one end of the fragment.
How do I get started?
You can start using MinION straight away.
For a $1,000 fee you receive a MinION and starter pack of flow cells and kits. You also get access to a thriving online community of MinION users.
What equipment and kits will I need?
You will need a MinION, flow cells and sequencing kits. These are provided in your starter pack. You can purchase additional flow cells and kits at any time; see the full range available here.
You will also need a laptop of the required specification and certain consumables. A range of frequently asked questions can be found here.