Fig. 3 Analysis of microbiome from soil around the roots of Basmati rice, shown at genus level
To demonstrate the effectiveness of WIMP for the analysis of a highly complex microbial community, we took a sample of soil from around the roots of a Basmati rice plant and extracted total genomic DNA by bead-beating. We prepared and sequenced an LSK-108 library and identified the organisms present with WIMP. The microbial community consisted largely of bacteria, though some fungal species were also identified (Fig. 3). WIMP identified many species of nitrogen-fixing bacteria, such as those belonging to the Bradyrhizobium and Rhizobium genera. Notably, WIMP also revealed the presence of several phytopathogens, including Xanthomonas translucens, which causes leaf streak, and can reduce crop yields substantially.
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