LAST

Martin Frith, Computational Biology Research Center in Tokyo

Release Date: 18-Sep-2015

LAST finds similar regions between sequences, and aligns them. It is designed for comparing large datasets to each other (e.g. vertebrate genomes and/or large numbers of DNA reads). It can:

• Indicate the (un)ambiguity of each column in an alignment
• Use sequence quality data in a rigorous fashion
• Align DNA to proteins with frameshifts
• Compare PSSMs to sequences
• Calculate the likelihood of chance similarities between random sequences
• Do split and spliced alignment