Nanopore analysis can be used to explore epigenetic modifications
The MinION - the portable, long read, real time sequencer – is used to sequence native DNA and RNA. Direct characterisation of these modifications is important whether researching cell differentiation or disease.
Being able to work with native DNA and RNA opens up opportunities to identify and characterise the wide range of modifications that exist.
The high-throughput, high sample-number, on-demand PromethION is now being released into early access.
If you are interested in epigenetics read these publications:
What's in my pot (WIMP)
'What's in my pot' (WIMP) is a workflow that enables MinION users to identify pathogens, fungi, viruses or archaea in complex samples in real time.
Analysis begins as soon as sequence data starts being streamed (a few seconds after the experiment starts). Each read of streamed sequence data is compared against a database of microbial species, and an identification is made. At the same time, WIMP plots and updates a taxonomic tree of all microorganisms found in the sample
There are various workflows and techniques that have been developed by members of the nanopore community to perform or enhance assembly and species identification that can be explored in the publications area and within the nanopore community
How do I get started?
You can start using MinION straight away.
For a $1,000 fee you receive a MinION and starter pack of flow cells and kits. You also get access to a thriving online community of MinION users.