Verkko: telomere-to-telomere assembly of diploid chromosomes
Here, Koren and colleagues present the assembly tool Verkko, which integrates "ultra-long reads" and "long, accurate reads" to generate high-quality, telomere-to-telomere human genome assemblies. ‘Running Verkko on the HG002 human genome resulted in 20 of 46 diploid chromosomes assembled without gaps at 99.9997% accuracy.’
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<i>De novo</i> sequencing, diploid assembly, and annotation of the black carpenter ant, Camponotus pennsylvanicus, and its symbionts by one person for $1000, using nanopore sequencing
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De novo assembly of Plasmodium knowlesi genomes from clinical samples explains the counterintuitive intrachromosomal organization of variant SICAvar and kir multiple gene family members
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In the Nanopore Community, customers raise discussions to connect with others working on the same things, or give guidance to other users. Current discussions include:
- Adaptive sampling on direct RNA (depletion)
- Plant virus cDNA optimization
- Direct RNA-seq: questions about splice variants for targeted gene
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Kit V14: now available in store
The latest Kit V14 chemistry, for Q20+ raw read accuracy, is now available to purchase from the store as part of an open, early access program. Kit 14 allows users to tune their accuracy and output needs by modifying run conditions — programmable in the software. Kit 14 has been shown to deliver simplex reads of Q20+ and is compatible with duplex reads, with data from both strands of the DNA molecule combined for single molecule accuracies of ~Q30.
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The genomic and epigenomic landscape of HPV in cervical cancer
Read how Vanessa Porter, University of British Columbia, Canada, and Michael Dean, National Cancer Institute, USA, are using nanopore sequencing to further our understanding of how HPV is driving cervical cancer transformation. From delineating complex integration events, through to methylation analysis, and chromatin conformation.
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