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Nanopore Digest: Telomere-to-telomere assembly of diploid chromosomes

30th June 2022

Verkko: telomere-to-telomere assembly of diploid chromosomes

Here, Koren and colleagues present the assembly tool Verkko, which integrates "ultra-long reads" and "long, accurate reads" to generate high-quality, telomere-to-telomere human genome assemblies. ‘Running Verkko on the HG002 human genome resulted in 20 of 46 diploid chromosomes assembled without gaps at 99.9997% accuracy.’

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<i>De novo</i> sequencing, diploid assembly, and annotation of the black carpenter ant, Camponotus pennsylvanicus, and its symbionts by one person for $1000, using nanopore sequencing

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De novo assembly of Plasmodium knowlesi genomes from clinical samples explains the counterintuitive intrachromosomal organization of variant SICAvar and kir multiple gene family members

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Community News

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Kit V14: now available in store

The latest Kit V14 chemistry, for Q20+ raw read accuracy, is now available to purchase from the store as part of an open, early access program. Kit 14 allows users to tune their accuracy and output needs by modifying run conditions — programmable in the software. Kit 14 has been shown to deliver simplex reads of Q20+ and is compatible with duplex reads, with data from both strands of the DNA molecule combined for single molecule accuracies of ~Q30.

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The genomic and epigenomic landscape of HPV in cervical cancer

Read how Vanessa Porter, University of British Columbia, Canada, and Michael Dean, National Cancer Institute, USA, are using nanopore sequencing to further our understanding of how HPV is driving cervical cancer transformation. From delineating complex integration events, through to methylation analysis, and chromatin conformation.

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News article: New method helps unravel three-dimensional structure of the human genome

In this article by Medical Life Sciences, read how Weill Cornell Medicine and New York Genome Center researchers, in collaboration with the Oxford Nanopore Technologies Applications Team, have developed a novel method, Pore-C, to unravel the three-dimensional structure of any genome.

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London Calling 2022: catch up with all the talks in your research area

Interested in catching up on all the talks from London Calling 2022 featuring your specific research area? You're in luck! We’ve put together several research area specific pages.

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