Basecalling your data and detecting methylation

Jessica Anderson, Technical Applications Scientist, gave her masterclass on how to basecall nanopore sequencing data, how to choose the right basecalling model, and how to call methylation in the data. This started with the basics of nanopore sequencing data analysis, including the different approaches available and file types involved, before moving on to the basecalling options available, and how to choose a basecalling model to suit the specifics of an experiment. Finally, Jessica outlined how to call methylation from PCR-free nanopore sequencing datasets, therefore detecting methylation directly from native DNA and RNA sequencing data.