Fig. 2 Direct RNA a) accuracy, b) alignment coverage, c) Circos plot, d) correlation with Illumina
We sequenced a whole-transcriptome RNA library prepared from Saccharomyces cerevisiae S228C. The modal read accuracy was > 90% (Fig. 2a). Mapping to the reference transcriptome and calculating the proportion of each transcript covered by each read shows that we obtain a high proportion of full-length reads (Fig. 2b). We obtained 100-base paired-end Illumina data of the same sample, aligned the Direct RNA and Illumina reads to the reference and calculated the log-fold difference in coverage (Fig. 2c). 2,045,748 (63.43%) Direct RNA reads mapped using GMAP 22, and 708,592,030 (98.22%) Illumina reads mapped using GSNAP. Direct RNA gene-coverage corresponds well with the Illumina results, with a correlation of 0.73 (Fig. 2d).