Solanum pennellii. (Tomato)

Recent updates in Oxford Nanopore technology (R9.4) have made it possible to obtain GBases of sequence data from a single flowcell. However, unlike other next generation sequencing technology, Oxford nanopore based sequencing doesn’t require any a priori capital investments. We therefore evaluated whether Oxford nanopore can be used to analyze plant genomes. To this aim, we sequenced and are assembling an accession of the wild tomato species Solanum pennellii. This accession was identified spuriously as an tomato accessions. Unlike the frequently used Solanum pennelii LA716 accession, for which we have previously generated a high quality draft genome, this new accession does not appear to exhibit any dwarfed, necrotic leaf phenotype when introgressed into modern tomato cultivars.

Authors: Institute for Botany and Molecular Genetics, BioEconomy Science Center, RWTH Aachen University, Aachen, Germany., Wageningen Plant Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands, Institute for Bio- and Geosciences (IBG-2: Plant Sciences), Forschungszentrum Jülich, Jülich, Germany., Department of Molecular Physiology, Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany., Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel., Genoscope (CEA) and UMR 8030 CNRS-Genoscope-Université d'Evry, 2 rue Gaston Crémieux, BP5706, 91057 Evry, France.