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Lorne Genome 2020

Sun 16th February - Tue 18th February 2020

Cumberland Lorne Resort, Lorne, Australia

Lorne Genome is the premier genomics research conference in Australasia and has a long tradition of hosting the world’s best researchers in  genomics. The Lorne Genome Conference is the premier interdisciplinary conference in Australia covering all areas of genome biology. Participant’s scientific interests range from clinical, disease-oriented and population genetics in humans, animals and plants, to fungal genetics and basic molecular genetics. This makes an ideal environment for students, researchers, and other professionals from within the industry to come together and discuss the latest in genome biology.
 

A breakfast seminar with Oxford Nanopore Technologies at Lorne Genome 2020

Date: Monday 17th February, 2020
Time: 7:00 - 8:40 am
Location: Cumberland Lorne Resort, VIC

Hear about the latest tech updates from Oxford Nanopore Technologies as well as talks from local scientists about their latest work using nanopore technology. 

Agenda below is subject to change:

07:00 – 07:30 – Registration & coffee

07:30 – 07:40 – Welcome & introduction, Mari Miyamoto, Oxford Nanopore Technologies Ltd.

07:40 – 08:00 – An update from Oxford Nanopore Technologies, Warren Bach, Oxford Nanopore Technologies Ltd.

08:00 – 08:20Direct RNA sequencing of host and pathogen using nanopore sequencing, Lachlan Coin, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne

08:20 – 08:35 – Q&A Panel: Warren Bach, Lachlan Coin, Michael Yarski

08:35 – 08:40 – Closing remarks, Mari Miyamoto

There is no additional fee for this event, but you must have a Lorne Genome delegate pass. Breakfast will be provided.
Your place at this event will be confirmed via email from events@nanoporetech.com. Completion of this form does not constitute confirmation. Spaces are limited and will be allocated on a first-come, first-served basis. Please complete the form below to apply for a place.

 

Direct RNA sequencing of host and pathogen using nanopore sequencing - Lachlan Coin, University of Melbourne

Direct RNA sequencing analysis is currently feasible using Nanopore sequencing and it has opened new frontiers in transcriptomic analysis. Long read sequencing combined with native RNA sample analysis provides opportunity to analyse known full-length transcripts, isoforms and discover novel transcripts and novel isoforms, which can be valuable for biomarker discovery. We utilized blood samples collected in PAXgene tubes to assess the utility of direct RNA sequencing for human transcriptomic analysis in clinical samples with limited input material. We used total RNA without polyA enrichment as input for Nanopore sequencing analysis and performed direct RNA sequencing and direct cDNA sequencing using Oxford Nanopore MinION sequencing. We also performed lllumina RNA sequencing on these samples.

We generated more than 300,000 reads per sample for direct RNA sequencing. Despite low coverage, more than 93% of the pass reads aligned to the human genome reference with an average identity of 87.6% for direct RNA sequencing. Nanopore direct RNA sequencing data was comparable to Illumina RNA sequencing data and the correlation values range between 0.84 – 0.97 for coding transcripts. Nanopore direct RNA and direct cDNA sequencing data also had higher correlation (0.958). We identified full-length transcripts and also identified novel transcripts and novel isoforms in direct RNA sequencing data, which were confirmed either by Nanopore direct cDNA or Illumina RNA sequencing data or by both.

We have successfully generated direct RNA sequencing data on RNA extracted from PAXgene tubes using Nanopore sequencing. Our results demonstrates it is feasible to generate direct RNA sequencing data with limited input material and also the outcome is comparable to cDNA sequencing data. We believe utilizing direct RNA sequencing for transcriptomic analysis has the potential to enhance our understanding of human transcriptomics and improve discovery of transcriptomic biomarkers.

Booth:
18

Event category:
Conferences

Type of Event:
Conference

Admission:
Public