Tue 20th June 2017
In this Knowledge Exchange, Dr Chris Vollmers, Assistant Professor at UC Santa Cruz, will explain how RNA from single B cells was sequenced using the Oxford Nanopore MinION device. The resulting full-length cDNA reads, with little-to-no length bias, were analysed to show the widespread isoform variation in the cells.
The presentation will cover:
- How the complex diversity of transcript isoforms is revealed by nanopore sequencing at a single cell level;
- The correlation of long-read gene expression profiling with that of traditional short read method;
- About Mandalorion, a new analysis pipeline for isoform detection even when using low amounts of mRNA;
- The presentation will be followed by online Q&As using the comments section below.
The BioRxiv paper for this work can be found here.
Dr Chris Vollmers, Assistant Professor at UC Santa Cruz
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