Tue 11th July 2017
Rapid and accurate diagnosis is critical for the effective treatment of life-threatening infections, such as bloodstream, respiratory tract and complicated urinary tract infections (UTIs). These clinical syndromes are difficult to diagnose due to complex aetiology and challenging clinical sample types (e.g. blood, sputum).
In this webinar, Justin O’Grady, PhD, will describe how he developed a MinION based infectious diseases diagnostics pipelines with examples from ongoing research on pneumonia, sepsis and UTIs.
Current culture based diagnosis often has sub-optimal specificity and sensitivity and is too slow to impact on patient management. Shotgun metagenomics sequencing has the potential to change the way we diagnose infection, combining rapidity with comprehensiveness beyond that of current methods. Real-time nanopore sequencing technology provides the rapid turnaround necessary for infectious diseases diagnostics applications.
There are challenges in applying sequencing to infection diagnosis, however, including high human: pathogen nucleic acid ratios, low pathogen numbers and low-quality nucleic acid, depending on the disease and the clinical sample type. It is, therefore, vital to carefully design, develop, and optimise diagnostics pipelines before attempting to apply them to clinical samples.
Justin O’Grady, PhD, Senior Lecturer at UEA
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