Whole Genome Sequencing
Nanopore sequencing offers comprehensive and rapid characterisation of whole genomes.
Latest news: On 1st December 2016, at the Nanopore Community Meeting in New York, the first human genomes to be sequenced on a MinION were presented. Find out more here.
Long reads, real-time data, <10 minute library prep and scalable technology all enable simple workflows, effective research and put you in control of your science with no capital investment required.
MinION and PromethION can be used for:
- de novo assembly
- analysis of structural variation
- analysis of single nucleotide variants and phasing
- phylogenetic relationships between species
- metagenomic analysis of environmental samples
- scaffolding and finishing of genomes
If you are planning to generate a new genome assembly, resolve repetitive regions, explore the importance of CNVs, SNPs or other variants in disease genotypes, or close gaps to complete a genome, nanopore sequencing produces the long-read data needed in your own lab with no capital investment required.
Whole genome sequencing on the MinION means:
Spanning difficult regions with long reads
Nanopores sequence the library strands presented to them, regardless of their length, rather than generating specific read lengths. Users can choose protocols which produce long reads of several kilobases to ultra-long reads of hundreds of kilobases of genomic DNA. This offers the opportunity to generate reads spanning areas which have not been addressed by shorter-read technologies; whether looking for clarity on haplotypes, a region containing a CNV or closing a gap.Spanning difficult regions
Efficient genome assembly
Long reads reduce the challenge of de novo assembly, resulting in fewer, longer contigs. This opens up the potential for more complete genome assemblies with fewer gaps.de novo assembly
Real-time data, rapid results
Nanopore sequencing data starts streaming immediately, rather than being delivered at the end of a 'run'. Real-time data streaming allows immediate analysis of this data allowing the researcher to monitor the amount of data being generated and when to stop the experiment. For metagenomic experiments, rapid identification of microorganisms in the sample is also possible using a number of tools. Oxford Nanopore provides a dedicated workflow 'WIMP Bacteria, Virus and Fungi', which gives real-time species identification and characterisation.Real-time species identification
How are others using the MinION for whole genome sequencing?
View these publications to find out more.