DNA: nanopore sequencing
DNA sequencing is the first application available on Oxford Nanopore systems; it is being performed by users of the handheld MinION device and is also compatible with the PromethION and future devices. A wide range of publications show the multiple applications that are being generated by the nanopore community.
'Strand sequencing' is a technique that passes intact DNA polymers through a protein nanopore, sequencing in real time as the DNA translocates the pore.
Library preparation is a simple 10-minute procedure that prepares for '1D' sequencing (sequencing one strand of the DNA). As data streams immediately, analyses can be performed during the experiment.
Alternatively, '2D' methods sequence both the template and complement strand of the DNA for two looks at the sequence.
MinION is available now for $1,000 for a starter kit.