DNA: nanopore sequencing
Oxford Nanopore systems offer real-time, scalable, direct DNA sequencing. This can be performed on the portable MinION device, the benchtop GridION and the high-thoughput, high-sample number PromethION.
Nanopore sequencing also offers, for the first time, direct RNA sequencing, as well as PCR or PCR-free cDNA sequencing.
With nanopore sequencing, the user chooses fragment length and the nanopore sequences the entire fragments. Reads approaching 1Mb have been reported.
'Strand sequencing' is a technique that passes intact DNA polymers through a protein nanopore, sequencing in real time as the DNA translocates the pore.
Library preparation is a simple 10-minute procedure that prepares for '1D' sequencing (sequencing one strand of the DNA) or '1D squared' an alternative method that can boost accuracy. As data streams immediately, analyses can be performed during the experiment.
Get started by buying a $1,000 MinION starter pack today.