The portable MinION offers portable, long read, real time DNA/RNA sequencing.
It is being used for a variety of genomic analysis techniques
What would you like to do with real-time long reads?
- Generate a complete de novo assembly
- Resolve and assess the impact of structural variants
- Sequence through repetitive sequences to understand their significance
- Explore SNPs across a targeted region
- Rapidly identify species in a mixed population or characterise RNA viruses and related epidemiology
- Study isoforms, splice variants and fusion proteins more clearly with full-length transcripts
- Work with native DNA to be able to explore epigenetic modifications
- Sequence and analyse RNA directly avoiding PCR and the use of reverse transcriptases
What's in my pot (WIMP)
'What's in my pot' (WIMP) is a workflow that enables MinION users to identify pathogens, fungi, viruses or archaea in complex samples in real time.
Analysis begins as soon as sequence data starts being streamed (a few seconds after the experiment starts). Each read of streamed sequence data is compared against a database of microbial species, and an identification is made. At the same time, WIMP plots and updates a taxonomic tree of all microorganisms found in the sample
There are various workflows and techniques that have been developed by members of the nanopore community to perform or enhance assembly and species identification that can be explored in the publications area and within the nanopore community
How do I get started?
You can start using MinION straight away.
For a $1,000 fee you receive a MinION and starter pack of flow cells and kits. You also get access to a thriving online community of MinION users.