Genome research and sequencing with nanopore technology
Reveal the ‘hidden’ genome with long nanopore sequencing reads. Fully characterise structural variation (SV), repetitive regions, single nucleotide variation (SNV), haplotype phasing, RNA splice variants, isoform usage, and fusion genes. In addition, identify base modifications alongside nucleotide sequence — with no additional sample prep requirements — through direct sequencing of native DNA or RNA.
- Resolve challenging genomic regions using ultra-long sequencing reads (up to 2 Mb)
- Sequence, characterise, and quantify full-length RNA transcripts for superior differential gene and isoform expression studies
- Eliminate bias and identify base modifications through direct sequencing of native DNA or RNA
- Get faster access to results with real-time data streaming – stop sequencing, store or reuse flow cell once result is obtained
- Streamline your workflow with 10-minute library prep (DNA)/105-minute (direct RNA)
- Scale to your needs using Flongle, MinION, GridION, or PromethION
How will you use nanopore technology?
Whole genome sequencing
Analysis of targeted regions
Gene expression and transcriptomics
Epigenetics
Nanopore sequencing does not require DNA amplification or fragmentation, enabling the generation of extremely long reads — resolve challenging genomic regions such as structural variants and repeat regions. Using nanopore sequencing, researchers have reported read lengths in excess of 2 Mb.
- Generate more complete prokaryotic or eukaryotic genome assemblies using long and ultra-long sequencing reads
- Accurately resolve structural variants and repeat regions
- Investigate linkage and haplotype phasing
- Detect epigenetic modifications alongside nucleotide sequence
- Scale to suit your research or lab requirements — 1.8 Gb Flongle; 30 Gb MinION; 150 Gb GridION; 4,800/9,600 Gb PromethION P24/P48
‘Nanopore sequencing is a tool that can let us look at things that we couldn’t otherwise see’
Dr. Winston Timp, Johns Hopkins University, USA
Long-read sequencing technologies resolve most dark and camouflaged gene regions
Long-read nanopore technology expands the application of targeted sequencing to enable comprehensive and cost-effective analysis of multiple large genomic regions of interest — including resolution of SVs, repetitive regions, SNVs, and phasing. Amplification-free CRISPR/Cas-mediated enrichment techniques further allow the simultaneous detection of base modifications alongside nucleotide sequence.
- Comprehensive characterisation — SVs, repeats, SNVs, phasing, and epigenetic modifications in a single assay
- Discover more — sequence entire genes or genomic regions in single, full-length reads
- Your choice of enrichment strategy — amplicon, hybrid-capture, CRISPR/Cas
- Detect base modifications and reduce bias using amplification-free approaches
- Rapid, streamlined workflows
- Real-time data streaming for immediate access to results
‘Nanopore [targeted] sequencing of CYP2D6 offers a high throughput method for genotyping, accurate haplotyping, and detection of new variants and duplicated alleles’
Targeted nanopore sequencing with Cas9 for studies of methylation, structural variants and mutations
The high yields of long, full-length sequencing reads delivered by nanopore technology enable complete transcriptome characterisation, including unambiguous identification and quantification of full-length transcript isoforms. Native RNA or cDNA can be analysed without fragmentation or amplification, streamlining analysis and removing potential sources of bias. Direct sequencing of native RNA further allows the identification of base modifications alongside nucleotide sequence.
- Complete characterisation and quantification of full-length transcripts
- Rapid and comprehensive analysis of RNA viruses
- Eliminate bias with amplification-free protocols
- Identify modified bases through direct RNA sequencing
- Access results in real-time
- Streamlined protocols with high sequencing yields
‘We generated a de novo transcriptome assembly of the olive fly and identified 3,553 novel genes and a total of 79,810 transcripts; a four-fold increase in transcriptome diversity compared to the NCBI predicted transcriptome’
Dissecting RNA biology, one molecule at a time
Unlike traditional sequencing techniques that require amplification and fragmentation, nanopore technology can analyse native DNA or RNA directly, preserving base modifications and allowing their detection alongside the nucleotide sequence. Long, full-length nanopore sequencing reads also enable phasing of all genomic variants — including modified bases, SVs, SNVs and repeats — providing complete genomic characterisation. Epigenetic modifications can be studied using both whole genome and targeted sequencing approaches.
- Detect modified bases alongside nucleotide sequence
- Phase DNA modifications and assign RNA modifications to specific isoforms
- Characterise full-length long non-coding RNA (lncRNA)
- Use whole genome or amplification-free targeted sequencing techniques
- Explore multi-way chromatin interactions using long reads
- Train basecalling algorithms to detect any base modification
‘With no additional sample preparation than that routinely used for basic sequencing and with only a mean coverage of ∼10×, we identify differential allelic methylation throughout the genome’
Targeted nanopore sequencing with Cas9 for studies of methylation, structural variants and mutations
Which kit is best for you?
