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Nanopore Digest: 3rd March 2020

Tue 3rd March 2020

Welcome to the Oxford Nanopore digest - a regular newsletter of updates from the nanopore community.  You'll be able to find links to recent publications, presentations and other news.  If you'd like to get the digest in your inbox please let us know.

Targeted nanopore sequencing with Cas9-guided adapter ligation

In this publication, Gilpatrick et al. describe their nanopore Cas9-Targeted Sequencing (nCATS) method. Focusing on 10 cancer genes, they used this technique to interrogate methylation and detect variants in cancer cell lines and tumour tissue samples. Long nanopore sequencing reads facilitated the identification of structural variants at target loci, and simplified the phasing of methylation into different alleles, allowing the team to investigate allele-specific epigenetic changes.

View the publication


Publications


Blog: Long reads reveal small-scale structural variations in an allo-tetraploid crop plant

Harmeet Singh Chawla, from Justus Liebig University Giessen, discusses how he developed a pipeline using long nanopore reads to investigate genomic structural variation in the major crop Canola.

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