Nanopore Digest: 28th February 2019
Thu 28th February 2019
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Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology
In this pre-print, Taylor et al. describe how they used the MinION to sequence the entire chromosome and plasmids of two foodborne pathogens, Salmonella enterica subsp. enterica serovar Bareilly and Escherichia coli O157:H7. Full-length genomes were obtained for both species, and the long-read assemblies were used to provide information on serotype, virulence factors, antimicrobial resistance genes, and phylogeny. Nanopore sequencing provided a shorter turnaround time compared to other sequencing technologies, achieving genome assembly within only four hours of sequencing, demonstrating its value in the rapid identification and characterisation of foodborne pathogens.
A guide to cDNA sequencing with Oxford Nanopore
Nanopore cDNA sequencing delivers millions of full-length transcripts enabling greater insight into the transcriptome.
Download our new cDNA sequencing guide to find out which kit is best for you and understand the process from sample to answer.
Ongoing discussions and questions in the Nanopore Community
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