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Nanopore Digest: 28th February 2019

Tue 5th March 2019

Salmonella bacteria

Welcome to the Oxford Nanopore digest - a regular newsletter of updates from the nanopore community.  You'll be able to find links to recent publications, presentations and other news.  If you'd like to get the digest in your inbox please let us know.

Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology

In this pre-print, Taylor et al. describe how they used the MinION to sequence the entire chromosome and plasmids of two foodborne pathogens, Salmonella enterica subsp. enterica serovar Bareilly and Escherichia coli O157:H7. Full-length genomes were obtained for both species, and the long-read assemblies were used to provide information on serotype, virulence factors, antimicrobial resistance genes, and phylogeny. Nanopore sequencing provided a shorter turnaround time compared to other sequencing technologies, achieving genome assembly within only four hours of sequencing, demonstrating its value in the rapid identification and characterisation of foodborne pathogens.

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A guide to cDNA sequencing with Oxford Nanopore

Nanopore cDNA sequencing delivers millions of full-length transcripts enabling greater insight into the transcriptome.

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