Nanopore Digest: 27th June 2019
Thu 27th June 2019
Welcome to the Oxford Nanopore digest - a regular newsletter of updates from the nanopore community. You'll be able to find links to recent publications, presentations and other news. If you'd like to get the digest in your inbox please let us know.
Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection
The current gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is culture, which is slow and lacks sensitivity. In this article published in Nature Biotechnology, Charalampous et al. describe a 6 hour sample preparation and nanopore sequencing workflow for the rapid and accurate characterisation of bacterial LRIs in respiratory samples. Achieving a maximum specificity and sensitivity of 100%, their method involves efficient saponin-based human DNA depletion, MinION sequencing, and real-time species identification and analysis using the “What’s in My Pot?” workflow.
Evolution of the bench
VolTRAX V2 is designed to enable users prepare samples for nanopore analysis hands-free, and without the need for a lab. Starter packs are available to buy now.
Ongoing discussions and questions in the Nanopore Community
Join the conversation and share your experiences.