Nanopore Digest: 20th July 2018
Fri 20th July 2018
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New tools for diet analyses: nanopore sequencing of metagenomic DNA from stomach contents to quantify diet in an invasive population of rats
Invasive species represent a catastrophic problem on native populations, and characterising the diet of these invaders is fundamental to understanding the impact they are having on both the environment and larger ecosystem. This information can then be used to inform control programmes.
Previous methods of diet characterisation include visual inspection, stable isotope analysis, timelapse video and DNA metabarcoding, but these all have challenges. In this paper, Pearman et al. show direct metagenomic nanopore sequencing allows rapid diet profiling, enabling pinpointing of the sampling location of a rat to within tens of kilometres.
Read case studies demonstrating the benefits of nanopore sequencing, from across the field of microbiology.
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