Nanopore Digest: 14th February 2019
Thu 14th March 2019
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Pooled CRISPR Inverse PCR sequencing (PCIP-seq): simultaneous sequencing of retroviral insertion points and the associated provirus in thousands of cells with long reads
Retroviral integration sites in the host genome have typically been detected by short-read sequencing, but this technology struggles to distinguish between full-length and fragmented provirus. Here the authors describe Pooled CRISPR Inverse PCR sequencing (PCIP-seq) which involves selectively cleaving circularised DNA fragments carrying proviral DNA with a pool of CRISPR guide RNAs, followed by inverse long-range PCR and nanopore sequencing. Nanopore long reads improved the accuracy of insertion site identification across repetitive regions of the genome and aided the efficient identification of tens of thousands of retroviral insertion sites.
Flongle now available in our store!
Flongle, an adapter for the portable MinION and desktop GridION X5 sequencing devices, is now available to buy in store. Flongle is designed to offer rapid insights at low cost to anyone, anywhere, any time.
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