Nanopore Digest: 14th February 2019
Thu 14th February 2019
Welcome to the Oxford Nanopore digest - a regular newsletter of updates from the nanopore community. You'll be able to find links to recent publications, presentations and other news. If you'd like to get the digest in your inbox please let us know.
Targeted, High-Resolution RNA Sequencing Of Non-Coding Genomic Regions Associated With Neuropsychiatric Functions
Genome wide association studies have identified thousands of haplotype blocks associated with neuropsychiatric traits. However, the majority of SNPs within these blocks tend to fall outside of genes. In this pre-print, Hardwick et al. performed targeted RNA capture (“RNA CaptureSeq”) from brain tissue followed by nanopore cDNA sequencing on the PromethION to profile 1,023 haplotype blocks associated with neuropsychiatric traits. Sixty-two percent of the blocks contained at least one novel multi-exonic transcript, many of which showed signs of potential functional roles. Unlike short-read sequencing, long reads obtained uniform exon coverage profiles and were able to detect splicing between distant exons, demonstrating their benefit for novel transcript assembly.
Webinar: The SG-NEx project: nanopore long-read RNA-sequencing of human cancer cell lines
Register for Jonathan Göke’s webinar exploring the use of cDNA sequencing in the SG-NEx project looking at human cancer cell lines, on Thursday 28th February 2019, 17:00 (GMT).
Find out how you can perform accurate isoform-level transcriptome characterisation and differential gene expression profiling with nanopore cDNA Kits.
Ongoing discussions and questions in the Nanopore Community
Join the conversation and share your experiences.